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Bioresource Technology • 2018

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Periodica Polytechnica Chemical Engineering • 2017

Acetic acid is an important platform chemical. It is mainly produced synthetically and only 10 percent of the world production is manufactured by bacterial fermentation for making vinegar. Several microorganisms can produce acetic acid and a part of them can incorporate CO2 during the production. In this review we summarized the microbial acetic acid pathways, and the used processes for vinegar production furthermore the optional acetic acid recovery operations to establish a complete possible biotechnological acetic acid production.

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ChemElectroChem • 2016

Abstract We demonstrate here the long‐term continuous bioelectrochemical chain elongation from CO 2 and acetate by using a mixed microbial culture. The role of applied current (3.1 vs. 9.3 A m −2 ) on the performance was investigated. The main product was n ‐butyrate which was continuously produced over time. Trace amounts of propionate and n ‐caproate were also produced, but no alcohols were detected during the whole course of the experiment (163 days). Microbial electrosynthesis (MES) systems controlled with more current (9.3 Am −2 ) showed a butyrate concentration that was 4.5 times higher (maximum 0.59 g L −1 ) and increased volumetric production rates (0.54 g L −1 day −1 ) compared to the low‐current reactors (0.12 g L −1 day −1 ), at 58.9 and 71.6 % electron recovery, respectively. Biocatalytic activity of the microbial consortia was demonstrated. This study revealed that the solid‐state electrode does control the chain elongation reaction as an essential electron donor and determines the performance of MES systems. This study highlights MES as a promising alternative for acetate upgrading.

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Chemical Society Reviews • 2022

fixation, we will discuss crucial factors for electrocatalysis with a special highlight on the improvement of electron-transfer kinetics, multi-dimensional electrocatalysts and their hybrids, electrolyser configurations, and the integration of electrocatalysis and bio-catalysis. Finally, we prospect key advantages and challenges of bio-electrocatalysis, and end with a discussion of future research directions.

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Water Research • 2020

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Biotechnology Advances • 2024

Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic changes has become an increasingly attractive approach for constructing robust microbial cell factories. In this review, we provide a brief overview of metabolic burden engineering, focusing specifically on recent developments and strategies for diminishing the burden while improving robustness and yield. A variety of examples are presented to showcase the promise of metabolic burden engineering in facilitating the design and construction of robust microbial cell factories. Finally, challenges and limitations encountered in metabolic burden engineering are discussed.

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Polymers • 2023

) adsorbents and membrane filtration technologies is highlighted to enhance adsorption and separation efficiency. The limitations and challenges associated are also discussed. The review concludes by emphasizing the importance of collaboration with industry stakeholders in advancing biopolymer-based nanocomposites for sustainable and comprehensive water treatment solutions.

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ACS Synthetic Biology • 2021

. We conclude by presenting challenges to future efforts and give examples of opportunities to bioengineer microbes for electrochemical applications.

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Environmental Research • 2021

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Bioresource Technology • 2016

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Renewable and Sustainable Energy Reviews • 2021

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Elsevier eBooks • 2020

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npj Science of Food • 2024

The global population is increasing, with a predicted demand for 1250 million tonnes of animal-derived protein by 2050, which will be difficult to meet. Single-cell protein (SCP) offers a sustainable solution. This review covers SCP production mechanisms, microbial and substrate choices, and advancements in metabolic engineering and CRISPR-Cas. It emphasizes second-generation substrates and fermentation for a circular economy. Despite challenges like high nucleic acid content, SCP promises to solve the global nutrition problem.

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Biosensors and Bioelectronics • 2015

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Biomedicines • 2020

Mitochondria are of great relevance to health, and their dysregulation is associated with major chronic diseases. Research on mitochondria-156 brand new publications from 2019 and 2020-have contributed to this review. Mitochondria have been fundamental for the evolution of complex organisms. As important and semi-autonomous organelles in cells, they can adapt their function to the needs of the respective organ. They can program their function to energy supply (e.g., to keep heart muscle cells going, life-long) or to metabolism (e.g., to support hepatocytes and liver function). The capacity of mitochondria to re-program between different options is important for all cell types that are capable of changing between a resting state and cell proliferation, such as stem cells and immune cells. Major chronic diseases are characterized by mitochondrial dysregulation. This will be exemplified by cardiovascular diseases, metabolic syndrome, neurodegenerative diseases, immune system disorders, and cancer. New strategies for intervention in chronic diseases will be presented. The tumor microenvironment can be considered a battlefield between cancer and immune defense, competing for energy supply and metabolism. Cancer cachexia is considered as a final stage of cancer progression. Nevertheless, the review will present an example of complete remission of cachexia via immune cell transfer. These findings should encourage studies along the lines of mitochondria, energy supply, and metabolism.

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Trends in biotechnology • 2020

Microbial electrochemical technologies (METs) are promising for sustainable applications. Recently, electron storage during intermittent operation of electroactive biofilms (EABs) has been shown to play an important role in power output and electron efficiencies. Insights into electron storage mechanisms, and the conditions under which these occur, are essential to improve microbial electrochemical conversions and to optimize biotechnological processes. Here, we discuss the two main mechanisms for electron storage in EABs: storage in the form of reduced redox active components in the electron transport chain and in the form of polymers. We review electron storage in EABs and in other microorganisms and will discuss how the mechanisms of electron storage can be influenced.

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Journal of CO2 Utilization • 2021

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Biotechnology for Biofuels • 2015

BACKGROUND: Volatile fatty acids (VFA) are building blocks for the chemical industry. Sustainable, biological production is constrained by production and recovery costs, including the need for intensive pH correction. Membrane electrolysis has been developed as an in situ extraction technology tailored to the direct recovery of VFA from fermentation while stabilizing acidogenesis without caustic addition. A current applied across an anion exchange membrane reduces the fermentation broth (catholyte, water reduction: H2O + e(-) → ½ H2 + OH(-)) and drives carboxylate ions into a clean, concentrated VFA stream (anolyte, water oxidation: H2O → 2e(-) + 2 H(+) + O2). RESULTS: In this study, we fermented thin stillage to generate a mixed VFA extract without chemical pH control. Membrane electrolysis (0.1 A, 3.22 ± 0.60 V) extracted 28 ± 6 % of carboxylates generated per day (on a carbon basis) and completely replaced caustic control of pH, with no impact on the total carboxylate production amount or rate. Hydrogen generated from the applied current shifted the fermentation outcome from predominantly C2 and C3 VFA (64 ± 3 % of the total VFA present in the control) to majority of C4 to C6 (70 ± 12 % in the experiment), with identical proportions in the VFA acid extract. A strain related to Megasphaera elsdenii (maximum abundance of 57 %), a bacteria capable of producing mid-chain VFA at a high rate, was enriched by the applied current, alongside a stable community of Lactobacillus spp. (10 %), enabling chain elongation of VFA through lactic acid. A conversion of 30 ± 5 % VFA produced per sCOD fed (60 ± 10 % of the reactive fraction) was achieved, with a 50 ± 6 % reduction in suspended solids likely by electro-coagulation. CONCLUSIONS: VFA can be extracted directly from a fermentation broth by membrane electrolysis. The electrolytic water reduction products are utilized in the fermentation: OH(-) is used for pH control without added chemicals, and H2 is metabolized by species such as Megasphaera elsdenii to produce greater value, more reduced VFA. Electro-fermentation displays promise for generating added value chemical co-products from biorefinery sidestreams and wastes.

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Frontiers in Microbiology • 2015

The mechanisms by which microbial species exchange electrons are of interest because interspecies electron transfer can expand the metabolic capabilities of microbial communities. Previous studies with the humic substance analog anthraquinone-2,6-disulfonate (AQDS) suggested that quinone-mediated interspecies electron transfer (QUIET) is feasible, but it was not determined if sufficient energy is available from QUIET to support the growth of both species. Furthermore, there have been no previous studies on the mechanisms for the oxidation of anthrahydroquinone-2,6-disulfonate (AHQDS). A co-culture of Geobacter metallireducens and G. sulfurreducens metabolized ethanol with the reduction of fumarate much faster in the presence of AQDS, and there was an increase in cell protein. G. sulfurreducens was more abundant, consistent with G. sulfurreducens obtaining electrons from acetate that G. metallireducens produced from ethanol, as well as from AHQDS. Co-cultures initiated with a citrate synthase-deficient strain of G. sulfurreducens that was unable to use acetate as an electron donor also metabolized ethanol with the reduction of fumarate and cell growth, but acetate accumulated over time. G. sulfurreducens and G. metallireducens were equally abundant in these co-cultures reflecting the inability of the citrate synthase-deficient strain of G. sulfurreducens to metabolize acetate. Evaluation of the mechanisms by which G. sulfurreducens accepts electrons from AHQDS demonstrated that a strain deficient in outer-surface c-type cytochromes that are required for AQDS reduction was as effective at QUIET as the wild-type strain. Deletion of additional genes previously implicated in extracellular electron transfer also had no impact on QUIET. These results demonstrate that QUIET can yield sufficient energy to support the growth of both syntrophic partners, but that the mechanisms by which electrons are derived from extracellular hydroquinones require further investigation.

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Processes • 2020

Currently over 845 million people are believed to be living under severe water scarcity, and an estimated 2.8 billion people across the globe are projected to come under serious water scarcity by the year 2025, according to a United Nations (UN) report. Seawater desalination has gained more traction as the solution with the most potential for increasing global freshwater supplies amongst other solutions. However, the economic and energy costs associated with the major desalination technologies are considered intrinsically prohibitive largely due to their humongous energy requirements alongside the requirements of complex equipment and their maintenance in most cases. Whilst forward osmosis (FO) is being touted as a potentially more energy efficient and cost-effective alternative desalination technique, its efficiency is challenged by draw solutes and the draw solutes recovery step in FO applications alongside other challenges. This paper looks at the present situation of global water scarcity, and a brief leap into the major desalination technologies employed. A closer look at the key drivers of FO as a seawater desalination technique in their individual domain and its outlook as an technology are further highlighted.

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RSC Advances • 2022

, domestic and industrial in the same system. In this case, contemporary advancement in MFC/MEC (METs) based systems towards Microbial-Electro-Fenton Technology (MEFT) revealed a substantial emerging scope and opportunity. Principally the said technology is based upon previously established anaerobic digestion and electro-chemical (photo/UV/Fenton) processes in the disciplines of microbial biotechnology and electro-chemistry. It holds an added advantage to all previously establish technologies in terms of treatment and energy efficiency, minimal toxicity and sludge waste, and environmental sustainable. This review typically described different dyes and their ultimate fate in environment and recently developed hierarchy of MEFS. It revealed detail mechanisms and degradation rate of dyes typically in cathodic Fenton system under batch and continuous modes of different MEF reactors. Moreover, it described cost-effectiveness of the said technology in terms of energy budget (production and consumption), and the limitations related to reactor fabrication cost and design for future upgradation to large scale application.

[object Object], [object Object], [object Object] et al.

Scientific Reports • 2015

Acetogens are efficient microbial catalysts for bioprocesses converting C1 compounds into organic products. Here, an adaptive laboratory evolution approach was implemented to adapt Sporomusa ovata for faster autotrophic metabolism and CO2 conversion to organic chemicals. S. ovata was first adapted to grow quicker autotrophically with methanol, a toxic C1 compound, as the sole substrate. Better growth on different concentrations of methanol and with H2-CO2 indicated the adapted strain had a more efficient autotrophic metabolism and a higher tolerance to solvent. The growth rate on methanol was increased 5-fold. Furthermore, acetate production rate from CO2 with an electrode serving as the electron donor was increased 6.5-fold confirming that the acceleration of the autotrophic metabolism of the adapted strain is independent of the electron donor provided. Whole-genome sequencing, transcriptomic, and biochemical studies revealed that the molecular mechanisms responsible for the novel characteristics of the adapted strain were associated with the methanol oxidation pathway and the Wood-Ljungdahl pathway of acetogens along with biosynthetic pathways, cell wall components, and protein chaperones. The results demonstrate that an efficient strategy to increase rates of CO2 conversion in bioprocesses like microbial electrosynthesis is to evolve the microbial catalyst by adaptive laboratory evolution to optimize its autotrophic metabolism.

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Molecules • 2020

conversion.

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Current Opinion in Biotechnology • 2016

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Nature Communications • 2023

Finding sustainable approaches to achieve independence from terrestrial resources is of pivotal importance for the future of space exploration. This is relevant not only to establish viable space exploration beyond low Earth-orbit, but also for ethical considerations associated with the generation of space waste and the preservation of extra-terrestrial environments. Here we propose and highlight a series of microbial biotechnologies uniquely suited to establish sustainable processes for in situ resource utilization and loop-closure. Microbial biotechnologies research and development for space sustainability will be translatable to Earth applications, tackling terrestrial environmental issues, thereby supporting the United Nations Sustainable Development Goals.

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Electrochemistry Communications • 2016

Platinum group metal-free (PGM-free) catalysts based on M-N-C types of materials with M as Mn, Fe, Co and Ni and aminoantipyrine (AAPyr) as N-C precursors were synthesized using sacrificial support method. Catalysts kinetics of oxygen reduction reaction (ORR) was studied using rotating ring disk electrode (RRDE) in neutral pH. Results showed that performances were distributed among the catalysts as: Fe-AAPyr > Co-AAPyr > Mn-AAPyr > Ni-AAPyr. Fe-AAPyr had the highest onset potential and half-wave potential. All the materials showed similar limiting current. Fe-AAPyr had an electron transfer involving 4e− with peroxide formed lower than 5%. Considering H2O2 produced, it seems that Co-AAPyr, Mn-AAPyr and Ni-AAPyr follow a 2 × 2e− mechanism with peroxide formed during the intermediate step. Durability test was done on Fe-AAPyr for 10,000 cycles. Decrease of activity was observed only after 10,000 cycles.

[object Object], [object Object], [object Object] et al.

ACS Synthetic Biology • 2018

(recombinant S. oneidensis), suggesting a boost in the EET efficiency. This modular engineering method in controlling the intracellular reducing equivalents would be a general approach in tuning the EET efficiency of exoelectrogens.

[object Object], [object Object], [object Object] et al.

mBio • 2018

-reductive methanogenesis in the environment, especially in sediments rich in conductive minerals. Mineral-facilitated SAO is therefore of potential importance for both iron and methane cycles in sediments and soils. Additionally, our observations imply that agricultural runoff or amendments with conductive chars could trigger a significant increase in methane emissions.

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Chemical Engineering Journal • 2018

This review presents the developments in the mathematical models for various bioelectrochemical systems. A number of modeling approaches starting with the simple description of biological and electrochemical processes in terms of ordinary differential equations to very detailed 2D and 3D models that study the spatial distribution of substrates and biomass, have been developed to study BES performance. Additionally, mathematical models focused on studying a particular process such as ion diffusion through membrane and new modeling approaches such as artificial intelligence methods, cellular network models, etc., have also been described. While most mathematical models are still focused on performance studies and optimization of microbial fuel cells, new models to study other BESs such as microbial electrolysis cell, microbial electrosynthesis and microbial desalination cell have also been reported and discussed in this review.

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Bioresource Technology • 2015

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Bioresource Technology • 2019

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Biotechnology for Biofuels • 2021

Non-model microorganisms often possess complex phenotypes that could be important for the future of biofuel and chemical production. They have received significant interest the last several years, but advancement is still slow due to the lack of a robust genetic toolbox in most organisms. Typically, "domestication" of a new non-model microorganism has been done on an ad hoc basis, and historically, it can take years to develop transformation and basic genetic tools. Here, we review the barriers and solutions to rapid development of genetic transformation tools in new hosts, with a major focus on Restriction-Modification systems, which are a well-known and significant barrier to efficient transformation. We further explore the tools and approaches used for efficient gene deletion, DNA insertion, and heterologous gene expression. Finally, more advanced and high-throughput tools are now being developed in diverse non-model microbes, paving the way for rapid and multiplexed genome engineering for biotechnology.

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FEMS Microbiology Reviews • 2015

Microbial electrochemical systems exploit the metabolism of microorganisms to generate electrical energy or a useful product. In the past couple of decades, the application of microbial electrochemical systems has increased from the use of wastewaters to produce electricity to a versatile technology that can use numerous sources for the extraction of electrons on the one hand, while on the other hand these electrons can be used to serve an ever increasing number of functions. Extremophilic microorganisms grow in environments that are hostile to most forms of life and their utilization in microbial electrochemical systems has opened new possibilities to oxidize substrates in the anode and produce novel products in the cathode. For example, extremophiles can be used to oxidize sulfur compounds in acidic pH to remediate wastewaters, generate electrical energy from marine sediment microbial fuel cells at low temperatures, desalinate wastewaters and act as biosensors of low amounts of organic carbon. In this review, we will discuss the recent advances that have been made in using microbial catalysts under extreme conditions and show possible new routes that extremophilic microorganisms open for microbial electrochemical systems.

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Renewable and Sustainable Energy Reviews • 2015

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New Biotechnology • 2017

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Frontiers in Microbiology • 2015

At deep-sea vent systems, hydrothermal emissions rich in reductive chemicals replace solar energy as fuels to support microbial carbon assimilation. Until recently, all the microbial components at vent systems have been assumed to be fostered by the primary production of chemolithoautotrophs; however, both the laboratory and on-site studies demonstrated electrical current generation at vent systems and have suggested that a portion of microbial carbon assimilation is stimulated by the direct uptake of electrons from electrically conductive minerals. Here we show that chemolithoautotrophic Fe(II)-oxidizing bacterium, Acidithiobacillus ferrooxidans, switches the electron source for carbon assimilation from diffusible Fe(2+) ions to an electrode under the condition that electrical current is the only source of energy and electrons. Site-specific marking of a cytochrome aa3 complex (aa3 complex) and a cytochrome bc1 complex (bc1 complex) in viable cells demonstrated that the electrons taken directly from an electrode are used for O2 reduction via a down-hill pathway, which generates proton motive force that is used for pushing the electrons to NAD(+) through a bc1 complex. Activation of carbon dioxide fixation by a direct electron uptake was also confirmed by the clear potential dependency of cell growth. These results reveal a previously unknown bioenergetic versatility of Fe(II)-oxidizing bacteria to use solid electron sources and will help with understanding carbon assimilation of microbial components living in electronically conductive chimney habitats.

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Journal of Cleaner Production • 2020

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International Journal of Hydrogen Energy • 2015

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Frontiers in Bioengineering and Biotechnology • 2020

, the energetic barriers have to be overcome. This review summarizes the pathway, the energetics of the pathway and describes ways to overcome energetic barriers in acetogenic C1 conversion.

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Polymers • 2021

Bioremediation is a key process for reclaiming polluted soil and water by the use of biological agents. A commonly used approach aims to neutralise or remove harmful pollutants from contaminated areas using live microorganisms. Generally, immobilised microorganisms rather than planktonic cells have been used in bioremediation methods. Activated carbon, inorganic minerals (clays, metal oxides, zeolites), and agricultural waste products are acceptable substrates for the immobilisation of bacteria, although there are limitations with biomass loading and the issue with leaching of bacteria during the process. Various synthetic and natural polymers with different functional groups have been used successfully for the efficient immobilisation of microorganisms and cells. Promise has been shown using macroporous materials including cryogels with entrapped bacteria or cells in applications for water treatment and biotechnology. A cryogel is a macroporous polymeric gel formed at sub-zero temperatures through a process known as cryogelation. Macroporous hydrogels have been used to make scaffolds or supports for immobilising bacterial, viral, and other cells. The production of composite materials with immobilised cells possessing suitable mechanical and chemical stability, porosity, elasticity, and biocompatibility suggests that these materials are potential candidates for a range of applications within applied microbiology, biotechnology, and research. This review evaluates applications of macroporous cryogels as tools for the bioremediation of contaminants in wastewater.