Jin Zhu, Baoguo Wang, Yixin Zhang et al.

Biosensors and Bioelectronics • 2023

Bioelectrical interfaces made of living electroactive bacteria (EAB) provide a unique opportunity to bridge biotic and abiotic systems, enabling the reprogramming of electrochemical biosensing. To develop these biosensors, principles from synthetic biology and electrode materials are being combined to engineer EAB as dynamic and responsive transducers with emerging, programmable functionalities. This review discusses the bioengineering of EAB to design active sensing parts and electrically connective interfaces on electrodes, which can be applied to construct smart electrochemical biosensors. In detail, by revisiting the electron transfer mechanism of electroactive microorganisms, engineering strategies of EAB cells for biotargets recognition, sensing circuit construction, and electrical signal routing, engineered EAB have demonstrated impressive capabilities in designing active sensing elements and developing electrically conductive interfaces on electrodes. Thus, integration of engineered EAB into electrochemical biosensors presents a promising avenue for advancing bioelectronics research. These hybridized systems equipped with engineered EAB can promote the field of electrochemical biosensing, with applications in environmental monitoring, health monitoring, green manufacturing, and other analytical fields. Finally, this review considers the prospects and challenges of the development of EAB-based electrochemical biosensors, identifying potential future applications.

Niloufar Fattahi, Jeffrey Reed, Evan Heronemus et al.

Bioelectrochemistry • 2024

Loss of bioelectrochemical activity in low resource environments or from chemical toxin exposure is a significant limitation in microbial electrochemical cells (MxCs), necessitating the development of materials that can stabilize and protect electroactive biofilms. Here, polyethylene glycol (PEG) hydrogels were designed as protective coatings over anodic biofilms, and the effect of the hydrogel coatings on biofilm viability under oligotrophic conditions and ammonia-N (NH 4 + -N) shocks was investigated. Hydrogel deposition occurred through polymerization of PEG divinyl sulfone and PEG tetrathiol precursor molecules, generating crosslinked PEG coatings with long-term hydrolytic stability between pH values of 3 and 10. Simultaneous monitoring of coated and uncoated electrodes co-located within the same MxC anode chamber confirmed that the hydrogel did not compromise biofilm viability, while the coated anode sustained nearly a 4 × higher current density (0.44 A/m 2 ) compared to the uncoated anode (0.12 A/m 2 ) under oligotrophic conditions. Chemical interactions between NH 4 + -N and PEG hydrogels revealed that the hydrogels provided a diffusive barrier to NH 4 + -N transport. This enabled PEG-coated biofilms to generate higher current densities during NH 4 + -N shocks and faster recovery afterwards. These results indicate that PEG-based coatings can expand the non-ideal chemical environments that electroactive biofilms can reliably operate in.

Yi Li, Weizhong Li, Yazhi Yang et al.

Biosensors and Bioelectronics • 2022

Mohamad Afiq Mohd Asrul, Mohd Farid Atan, Hafizah Abdul Halim Yun et al.

International Journal of Hydrogen Energy • 2025

Thi Pham Phan, Quang Huy Hoang Phan, Phan Khanh Thinh Nguyen

Fuel • 2024

Hong-zhou Liu, Tie-zhu Chen, Jian-chang Li

Journal of Power Sources • 2025

Xiaochi Zheng, Long Chen, Shaohui Zhang et al.

Journal of Water Process Engineering • 2024

Peike Wang, Min Zhang, Tianyu Yan et al.

Renewable Energy • 2025

Matthew J. Berens, Tobin W. Deen, Chan Lan Chun

Chemosphere • 2024

Zhang Cheng, Shiyun Yao, Heyang Yuan

Water Research • 2021

Mechanistic and data-driven models have been developed to provide predictive insights into the design and optimization of engineered bioprocesses. These two modeling strategies can be combined to form hybrid models to address the issues of parameter identifiability and prediction interpretability. Herein, we developed a novel and robust hybrid modeling strategy by incorporating microbial population dynamics into model construction. The hybrid model was constructed using bioelectrochemical systems (BES) as a platform system. We collected 77 samples from 13 publications, in which the BES were operated under diverse conditions, and performed holistic processing of the 16S rRNA amplicon sequencing data. Community analysis revealed core populations composed of putative electroactive taxa Geobacter, Desulfovibrio, Pseudomonas, and Acinetobacter. Primary Bayesian networks were trained with the core populations and environmental parameters, and directed Bayesian networks were trained by defining the operating parameters to improve the prediction interpretability. Both networks were validated with Bray-Curtis similarly, relative root-mean-square error (RMSE), and a null model. A hybrid model was developed by first building a three-population mechanistic component and subsequently feeding the estimated microbial kinetic parameters into network training. The hybrid model generated a simulated community that shared a Bray-Curtis similarity of 72% with the actual microbial community at the genus level and an average relative RMSE of 7% for individual taxa. When examined with additional samples that were not included in network training, the hybrid model achieved accurate prediction of current production with a relative error-based RMSE of 0.8 and outperformed the data-driven models. The genomics-enabled hybrid modeling strategy represents a significant step toward robust simulation of a variety of engineered bioprocesses.

Jiaohui Xia, Dan Chen, Cheng Hou et al.

Bioresource Technology • 2021

Nitrogen removal based on short-cut nitrification (SCN) have attract more attentions, in which stable nitrite accumulation is prerequisite. In this study, different reductive potential was applied to inhibit nitrite oxidizing bacteria for achievement of SCN in aerobic cathode chamber of bioelectrochemical systems with dissolved oxygen concentration of 3.5 mg/L. The results demonstrated that the applied potential facilitated nitrite accumulation with high ammonia oxidation rates. The maximum nitrate accumulation rate of 87.61% was obtained at -800 mV. The abundance of Nitrosomonas and Thauera increased while Nitrospira abundance declined with more negative reductive potentials. The activity of nitric oxide reductase was also evidently inhibited. The above-mentioned three genera were the keystone taxa in co-occurrence network with high degree and closeness centrality. Interestingly, total nitrogen (TN) removal was enhanced simultaneously in the absence of external organic carbon. Reductive potential would be a promising approach for achieving SCN and simultaneously TN removal.

V. S. Bueschler, G. V. Sayoga, H. Beisch et al.

Chemie Ingenieur Technik • 2022

Lili Tian, Chengmei Liao, Xuejun Yan et al.

Journal of Hazardous Materials • 2023

G. V. Sayoga, V. S. Bueschler, H. Beisch et al.

Chemie Ingenieur Technik • 2022

Tatiana Kuleshova, Elizaveta Ezerina, Vitaly Vertebny et al.

International Journal of Hydrogen Energy • 2024

Hesamoddin Rabiee, Lei Ge, Shihu Hu et al.

Chemical Engineering Journal • 2022

Bo Cao, Ting Zhao, Yanhong Ge et al.

Chemical Engineering Journal • 2025

Yolina Hubenova, Galin Borisov, Evelina Slavcheva et al.

Bioelectrochemistry • 2022

A novel strain of Gram-positive bacteria Paenibacillus profundus YoMME was recognized by sequencing of 16S rRNA gene and after that tested for exoelectrogenicity for the first time. It was found that at an applied potential of -0.195 V (vs. SHE) the bacteria are capable of generating electricity and forming electroactive biofilms for 3-4 days. A tendency for the decrease in double-layer capacitance and the increase in the charge transfer resistance during the maturation of the biofilm was established. The formed bioanodes were used as a part of a membrane-electrode assembly (MEA) together with a selected cathode (E-Tek) and a separator (Zirfon). The applicability of MEA with the bioanode was tested by operating a newly designed bioelectrochemical system in a microbial fuel cell (MFC) or microbial electrolysis cell (MEC) mode. A current density of 200 mA m -2 was generated by the MFC after the improvement of the cathodic reaction through facilitated air access. The Coulombic efficiency in different MFC runs ranged from 5.2 to 7.4%. It was also determined that 0.65 V applied cell voltage is appropriate for the operation of the cell in the electrolysis mode, during which a current density of 2-3 Am -2 was reached. This, along with the evolved gas on the cathode, shows that as an anodic biocatalyst P. profundus YoMME assists the electrolysis processes at a significantly lower voltage than the theoretical one (1.23 V) for water decomposition. The hydrogen production rate varied between 0.5 and 0.7 m 3 /m 3 d and the cathodic hydrogen recovery ranged from 49.5 to 61.5 %. The estimated energy efficiency based on the electricity input exceeds 100 %, which indicates that additional energy is being gained from the biotic oxidation of the available organics.

Jiawei Xie, Xinyi Zou, Yaofeng Chang et al.

Bioresource Technology • 2021

Fei Guo, Jerome T. Babauta, Haluk Beyenal

Bioresource Technology • 2021

Gaurav Kalia, Shashwat Mishra, Paramjeet Singh et al.

Materials Today: Proceedings • 2022

Zhen Fang, Jiani Hu, Meng-Yuan Xu et al.

Bioelectrochemistry • 2024

Bidirectional electron transfer is about that exoelectrogens produce bioelectricity via extracellular electron transfer at anode and drive cytoplasmic biochemical reactions via extracellular electron uptake at cathode. The key factor to determine above bioelectrochemical performances is the electron transfer efficiency under biocompatible abiotic/biotic interface. Here, a graphene/polyaniline (GO/PANI) nanocomposite electrode specially interfacing exoelectrogens (Shewanella loihica) and augmenting bidirectional electron transfer was conducted by in-situ electrochemical modification on carbon paper (CP). Impressively, the GO/PANI@CP electrode tremendously improved the performance of exoelectrogens at anode for wastewater treatment and bioelectricity generation (about 54 folds increase of power density compared to blank CP electrode). The bacteria on electrode surface not only showed fast electron release but also exhibited high electricity density of extracellular electron uptake through the proposed direct electron transfer pathway. Thus, the cathode applications of microbial electrosynthesis and bio-denitrification were developed via GO/PANI@CP electrode, which assisted the close contact between microbial outer-membrane cytochromes and nanocomposite electrode for efficient nitrate removal (0.333 mM/h). Overall, nanocomposite modified electrode with biocompatible interfaces has great potential to enhance bioelectrochemical reactions with exoelectrogens.

, Se Min Kim, Rajkumar Patel et al.

Membrane Journal • 2021

Harsha Nagar, Vineet Aniya, Ch. Saranya

Journal of Environmental Chemical Engineering • 2021

Sudipta Mondal, Farhan Papiya, Saumendra Nath Ash et al.

Journal of Environmental Chemical Engineering • 2021

Mahdi Shahparasti, Amirhossein Rajaei, Andres Tarrassó et al.

Energy • 2022

Wenbin Liu, Leiming Lin, Yukang Qie et al.

Resources, Conservation and Recycling • 2022

Yaping Zhang, Yangao Xu, Xi Chen et al.

Chemosphere • 2021

Toxic metal ions were previously found to be effective removed by anodic biofilms under the coexistence of organics in bioelectrochemical system (BES). However, the effect of toxic metal ions on the organics fermentation pathways remains unclear. To investigate the pathway systematically, the glucose fermentation pathways were discussed under different Cu 2+ concentrations. After introducing Cu 2+ , more acetate and less propionate were observed, implying that the metabolic reaction of glucose fermentation altered from mixed acid type to acetogenesis type. This pattern produced more "food" (acetate or hydrogen) for methanogens, thus, the methane content increased by 19.67%, 39.51%, and 27.71% in the presence of 0.1, 1, and 7 mg L -1 Cu 2+ compared to the blank, respectively. Increased Cu 2+ concentrations resulted in the decrease of current production, which was associated with the decrease of electricigen (Geobacter). Consistent with the change of fermentation type, the fermenters (Klebsiella and norank_f__norank_o__Bacteroidales) that related to the production of acetate increased, while the dominant methanogens (Methaospirillum) didn't decrease until the Cu 2+ concentration reached 7 mg L -1 .

Wei Wang, Jo-Shu Chang, Duu-Jong Lee

Bioresource Technology • 2022

Strategies for enhancing performance of anaerobic digestion (AD) process has been widely studied. The bioelectrochemical system (BES), including microbial fuel cell, microbial electrolysis cell (MEC), microbial desalination cell, and microbial electrosynthesis, had been proposed to integrate with AD for performance enhancement. This mini-review summarizes the current researches that integrated AD with BES to enhance the performance of the former. The working principles of BES were introduced. The integrated configurations of AD-BES as well as the associated applications were summarized. The statistics analysis for AD-MEC performances reported in literature were then performed to confirm the effects of reactor size and applied voltage on the methane productivity and enhancement. The challenges and prospects of the integrated AD-BES were delineated, and the potential scenarios of applying integrated AD-BES in field were discussed.

Yi-Xuan Wang, Xiao-Li Liu, Wen-Qiang Li et al.

Water Research • 2024

Muhammet Güler, Adem Zengin, Murat Alay

Analytical Biochemistry • 2023

Zhen Fang, Qichao Fan, Jiani Hu et al.

Journal of Environmental Chemical Engineering • 2025

Tingli Ren, Yuanfeng Liu, Congju Li

Chinese Chemical Letters • 2025

Boris Tartakovsky, Frédérique Lebrun, Serge R. Guiot et al.

Sustainable Energy Technologies and Assessments • 2021

Mihai-Cristian Fera, Rita R. Manuel, Inês A.C. Pereira et al.

Carbon • 2023

Ramineh Rad, Tito Gehring, Kevinjeorjios Pellumbi et al.

Cell Reports Physical Science • 2023

René Cardeña, László Koók, Jan Žitka et al.

Bioresource Technology • 2021

This work characterizes and comparatively assess two cation exchange membranes (PSEBS SU22 and CF22 R14) and one bipolar membrane (FBM) in microbial electrolysis cells (MEC), fed either by acetate or the mixture of volatile fatty acids as substrates. The PSEBS SU22 is a new, patent-pending material, while the CF22 R14 and FBM are developmental and commercialized products. Based on the various MEC performance measures, membranes were ranked by the EXPROM-2 method to reveal which of the polymeric membranes could be more beneficial from a complex, H 2 production efficiency viewpoint. It turned out that the substrate-type influenced the application potential of the membranes. Still, in total, the PSEBS SU22 was found competitive with the other alternative materials. The evaluation of MEC was also supported by analyzing anodic biofilms following electroactive bacteria's development over time.

Kaliaperumal Keruthiga, Samsudeen Naina Mohamed, Nagarajan Nagendra Gandhi et al.

International Journal of Hydrogen Energy • 2021

K. Keruthiga, N. Samsudeen, N. Nagendra Gandhi et al.

Journal of The Institution of Engineers (India): Series E • 2023

Uriel Roberto Pedroza-Medina, Luis Felipe Cházaro-Ruiz, Ricardo Femat et al.

Chemical Engineering and Processing - Process Intensification • 2024