Shinojus Lovelin Golda Anitus, Anchana Devi Chenniappan, Alison Christina Fernandez et al.

Asian Journal of Biological and Life Sciences • 2025

Mohamad Afiq Mohd Asrul, Mohd Farid Atan, Hafizah Abdul Halim Yun et al.

International Journal of Hydrogen Energy • 2021

Amit Kumar Chaurasia, Ravi Shankar, Prasenjit Mondal

Journal of Environmental Management • 2021

Jayachitra Murugaiyan, Anantharaman Narayanan, Samsudeen Naina Mohamed

International Journal of Energy Research • 2022

Abhispa Bora, K. Mohanrasu, T. Angelin Swetha et al.

Fuel • 2022

Gabriel Khew Mun Hong, Mohd Azlan Hussain, Ahmad Khairi Abdul Wahab

Chinese Journal of Chemical Engineering • 2021

Divyanshu Sikarwar, Indrasis Das, Anusha Ganta et al.

Sustainable Chemistry for the Environment • 2025

Hyung-Sool Lee, Wang Xin, Ranaprathap Katakojwala et al.

Bioresource Technology • 2022

To assess biohydrogen for future green energy, this review revisited dark fermentation and microbial electrolysis cells (MECs). Hydrogen evolution rate in mesophilic dark fermentation is as high as 192 m 3 H 2 /m 3 -d, however hydrogen yield is limited. MECs are ideal for improving hydrogen yield from carboxylate accumulated from dark fermentation, whereas hydrogen production rate is too slow in MECs. Hence, improving anode kinetic is very important for realizing MEC biohydrogen. Intracellular electron transfer (IET) and extracellular electron transfer (EET) can limit current density in MECs, which is proportional to hydrogen evolution rate. EET does not limit current density once electrically conductive biofilms are formed on anodes, potentially producing 300 A/m 2 . Hence, IET kinetics mainly govern current density in MECs. Among parameters associated with IET kinetic, population of anode-respiring bacteria in anode biofilms, biofilm density of active microorganisms, biofilm thickness, and alkalinity are critical for current density.

A. Saravanan, S. Karishma, P. Senthil Kumar et al.

Biomass Conversion and Biorefinery • 2023

Tamilmani Jayabalan, Manickam Matheswaran, T.K. Radhakrishnan et al.

Bioresource Technology • 2021

Biohydrogen production in Microbial Electrolysis Cell (MEC) had inspired the researchers to overcome the challenges associated towards sustainability. Despite microbial community and various substrates, economical cathode catalyst development is most significant factor for enhancing hydrogen production in the MEC. Hence, in this study, the performance of MEC was investigated with a sugar industry effluent (COD 4200 ± 20 mg/L) with graphite anode and modified Nickel foam (NF) cathode. Nickel molybdate (NiMoO 4 ) coated NF achieved a higher hydrogen production rate 0.12 ± 0.01 L.L -1 D -1 as compared to control under favorable conditions. Electrochemical characterizations demonstrated that the improved catalytic activity of novel nanocatalyst with lower impedance favoring faster hydrogen evolution kinetics. The MEC with the novel catalyst performed with 58.2% coloumbic efficiency, 20.36% cathodic hydrogen recovery, 11.96% overall hydrogen recovery and 54.38% COD removal efficiency for a 250 mL substrate during 5 days' batch cycle. Hence, the potentiality of modified cathode was established with the real time industrial effluent highlighting the waste to wealth bio-electrochemical technology.

Moeen Gholami, Behrooz Abbasi Souraki, Abbas Shomali et al.

Environmental Technology • 2024

ABSTRACT In the present study, a bioelectrochemical reactor (BEC) was utilized to treat two types of real saline produced water (PW). BEC was designed based on the combination of electrocoagulation (EC) process with halophilic microorganisms, and it was assessed in terms of biodegradation of hydrocarbons. The effects of various operating parameters including the current density, electrical contact time (On/Off), hydraulic retention time (HRT), and total dissolved solids (TDS) at different levels on the chemical oxygen demand (COD) removal efficiency, settleability, and performance of isolated halophilic microorganisms were examined. Additionally, a novel neural network (ANN) approach modelling using adaptive factors was used to predict and optimize the effects and interactions between operating parameters during BEC process by predicting complicated mechanisms and variations associated with microorganisms. In addition, a new algorithm was developed for the sensitivity analysis to achieve the optimum operating conditions and obtain maximum efficiency in COD removal, sludge volume index (SVI), mixed liquor suspended solids (MLSS), and specific electrical energy consumption (SEEC), simultaneously. BEC was found to be significantly more effective at removing most hydrocarbons, particularly pristine and phytane. In addition, the results showed a significant improvement in settling ability of the biological flocs with average SVI of 91.5 mL/g and a size of 178.25 μm using BEC. Based on estimated operating costs and energy consumption, BEC was more cost-effective and efficient than other bioelectrochemical systems.

Anis Askari, Milad Taherkhani, Farzaneh Vahabzadeh

Korean Journal of Chemical Engineering • 2022

Nuan Yang, Guoqiang Zhan, Huiqin Luo et al.

Bioresource Technology • 2021

Simultaneous nitrification/denitrification (SND) can efficiently deplete NH 4 + by using air-exposed biocathode (AEB) in bioelectrochemical reactors. However, the fluctuation of wastewater adversely affects the functional biofilms and therefore the performance. In this work, four up-flow bioelectrochemical reactors (UBERs) with some novel inocula were investigated to improve domestic wastewater treatment. The UBERs exhibited favorable removal of chemical oxygen demand (COD, 95%), NH 4 + -N (99%), and total nitrogen (TN, 99%). The maximum of current (2.7 A/m 3 ), power density (136 mW/m 3 ) and coulombic efficiency (20.5%) were obtained. Cyclic voltammetry analysis showed all the electrodes were of diversified catalytic reactions. Illumina pyrosequencing showed the predominant Ignavibacterium, Thauera, Nitrosomonas, Geminicoccus and Nitrospira were in all electrodes, contributing functional biofilms performing SND, comammox, and bioelectrochemical reactions. FAPROTAX analysis confirmed twenty-one functional groups with obvious changes related to chemoheterotrophy, respiration/oxidation/denitrification of nitrite and nitrate. Comfortingly, such novel diversified consortia in UBERs enhance the microbial metabolisms to treat domestic wastewater.

Vafa Ahmadi, Carlos Dinamarca, Nabin Aryal

Results in Engineering • 2025

Xavier Alexis Walter, Anastasiia Kostrytsia, Helen Watson et al.

Bioresource Technology • 2024

Florence de Fouchécour, Valentin Larzillière, Théodore Bouchez et al.

Water Research • 2022

Mehran Janmohammadi, Baiqian Shi, Tanveer M. Adyel et al.

Journal of Environmental Chemical Engineering • 2025

Haitai Dong, Xingzu Wang, Shun Lu et al.

Polymer Degradation and Stability • 2023

Lei Xu, Wenzheng Yu, Nigel Graham et al.

Chemosphere • 2021

Bioelectrochemical system (BES) based biosensors for organic sensing has long been investigated. However, there is no uniform criterion to evaluate directly the performance of the BES based biosensors due to their different scale. Here, for the first time, we show that the normalized maximum detection range (NMDR) and normalized sensing time (NST) can potentially be used as the two criteria in BES based biosensors for organic sensing. Thereafter, the recently emerged, relatively larger scale BES (i.e. constructed wetland-microbial fuel cell, CW-MFC) was specifically examined in this study. The biocathode formation and the influence of anodic material on sensor performance were systematically evaluated. The system with metal-based anode was found to produce a more stable and quicker response (low NST) than that with carbon-based anode. Significantly, the continuous loading mode was found to greatly reduce the NMDR compared to the batch mode, and the hydraulic residence time (HRT) is the critical factor determining the NMDR. Furthermore, it was found that the electrical signals generated from the CW-MFC system were insignificantly influenced by some specific chemical disturbances, such as Cu 2+ and herbicide. Therefore, normalized toxicity (NT) is suggested to be considered in BES based biosensor. However, for chemicals with higher reduction potentials (NO 3 - in this work), the system presented a high response, enabling its potential for monitoring NO 3 - in effluents or groundwater. This study can hopefully contribute to further development of the sustainable BES based biosensors in CW.

Anis Askari, Farzaneh Vahabzadeh, Mohammad Mahdi Mardanpour

Journal of Cleaner Production • 2021

Negin Naghibi, Moj Khaleghi, Seyed Ahmad Ataei et al.

Journal of Water Process Engineering • 2025

Ademola Adekunle, Carrie Rickwood, Boris Tartakovsky

Ecotoxicology • 2021

Real-time biomonitoring using microbial fuel cell (MFC) based biosensors have been demonstrated in several laboratory studies, but field validation is lacking. This study describes the long-term performance of an MFC based biosensor developed for real-time monitoring of changes in the water quality of a metal-contaminated stream. After a startup in the laboratory, biosensors were deployed in a stream close to an active mining complex in Sudbury, ON, Canada. Three sites within the stream were selected for biosensors installation based on their positions relative to the mining complex discharge points - upstream (lowest heavy metals concentration), midpoint and downstream. The biosensors installed at these sites were able to detect, in real-time, temporal changes in the water quality over a 2-month period. The biosensor response was confirmed by the results of a conventional toxicity assay (48-h acute Daphnia magna) as well as analytical measurements of heavy metals concentration in the stream. We conclude that the biosensor could detect changes in the overall water quality of the stream despite the uncontrolled situations typical for field operations as compared to laboratory conditions. To further explain the results observed during the field test, the rapid Microtox bioassay and D. magna assay were used to investigate the possible contributions of the two dominant mining metals (Nickel and Copper) to water toxicity in the test area.

Trang Nakamoto, Dung Nakamoto, Kozo Taguchi

Biochemical Engineering Journal • 2023

Xiao-Li Yang, Qi Wang, Tao Li et al.

Bioresource Technology • 2022

Antibiotics removal and ARGs control in microbial fuel cell (MFC) has received extensive attention. In particular, the critical role of bioelectrochemical characteristics deserves further study. Bioelectrochemical characteristics significantly affected sulfamethoxazole (SMX) removal and ARGs fate, in which the current intensity played a more critical role than anode potential. High-concentration SMX (2 mg/L and 10 mg/L) facilitated the anode potential tend to be close, and thus, the strengthening effect of current on the system was highlighted. However, the SMX degradation pathway under different bioelectrochemical characteristics was not affected. Furthermore, the higher current intensity was preferable to antibiotic removal, but unfavorable for ARGs control might be due to the oxidative stress on microorganisms. Low-concentration SMX (0.5 mg/L) contributed to improving higher electricity generation because of Geobacter enrichement. This study suggested that appropriate bioelectrochemical characteristics regulation in MFCs was essential in removing antibiotics and controlling ARGs.

Ke Zhang, Tingting Wang, Hongbing Luo et al.

Journal of Water Process Engineering • 2023

Qian Zhao, Zhen Hu, Jian Zhang et al.

Journal of Hazardous Materials • 2023

Chloramphenicol (CAP) has a high concentration and detection frequency in aquatic environments due to its insufficient degradation in traditional biological wastewater treatment processes. In this study, bioelectrochemical assistant-constructed wetland systems (BES-CWs) were developed as advanced processes for efficient CAP removal, in which the degradation and transfer of CAP and the fate of antibiotic resistance genes (ARGs) were evaluated. The CAP removal efficiency could reach as high as 90.2%, while the removed CAP can be partially adsorbed and bioaccumulated in plants, significantly affecting plant growth. The vertical gene transfer and horizontal gene transfer increased the abundance of ARGs under high voltage and CAP concentrations. Microbial community analysis showed that CAP pressure and electrical stimulation selected the functional bacteria to increase CAP removal and antibiotic resistance. CAP degradation species carrying ARGs could increase their opposition to the biotoxicity of CAP and maintain system performance. In addition, ARGs are transferred into the plant and upward, which can potentially enter the food chain. This study provides an essential reference for enhancing antibiotic degradation and offers fundamental support for the underlying mechanism and ARG proliferation during antibiotic biodegradation.

Ke Zhao, Shenghe Liu, Yimeng Feng et al.

Science of The Total Environment • 2024

Ercheng Luo, Guishi Cheng, Yanchang Liu et al.

Journal of Power Sources • 2025

Hegazy Rezk, Abdul Ghani Olabi, Mohammad Ali Abdelkareem et al.

International Journal of Energy Research • 2022

Santiago T Boto, Lorenzo Cristiani, Miriam A Rosenbaum

Current Opinion in Biotechnology • 2025

Microbial bioelectrochemical systems (BES) represent a promising platform for sustainable biochemical production by leveraging microbial electrocatalysis. These systems utilize electrical energy to drive microbial metabolic processes, enabling the recovery of CO₂ into valuable organic molecules such as methane, acetate, ethanol, and other biochemicals. This approach aligns with global efforts to mitigate greenhouse gas emissions and create circular carbon economies. The advancement of BES technology requires both scale-down and scale-up strategies to ensure feasibility and scalability. Scale-down approaches focus on optimizing operational parameters, enhancing electron transfer efficiencies, and understanding microbial community dynamics under controlled conditions. Scale-up efforts address the challenges of maintaining system stability, energy efficiency, and economic viability in larger, industrial-scale operations. Together, these strategies bridge the gap between fundamental laboratory research and real-world applications, positioning microbial BES as a key technology for sustainable biochemical production and captured carbon utilization.

Ioannis A. Ieropoulos, Aradhana Singh, Daniela Zertuche Moreno et al.

Current Opinion in Electrochemistry • 2024

Zhiming Zhang, Dibyendu Sarkar, Liang Li et al.

Current Pollution Reports • 2022

Christina F Webster, Won-Jun Kim, Gemma Reguera et al.

Current Opinion in Biotechnology • 2024

Prakash C. Sahoo, Deepak Pant, Manoj Kumar et al.

Current Opinion in Electrochemistry • 2024

Xinxin Xiao, Xiaomei Yan, Jens Ulstrup

Current Opinion in Electrochemistry • 2022

Laura Mais, Jesus Rodriguez, Nicola Melis et al.

Current Opinion in Electrochemistry • 2024

Kamonwan Khanthong, Heewon Jang, Rahul Kadam et al.

Chemosphere • 2023

Biological nitrogen removal (BNR) is essential for the treatment of nitrogen-containing wastewater. However, the requirement for aeration and the addition of external carbon sources, resulting in greenhouse gas emissions and additional costs, are disadvantages of the traditional BNR process. Alternative technologies have been devised to overcome these drawbacks. Bioelectrochemical nitrogen removal (BENR) has been proposed for efficient nitrogen removal, demonstrating flexibility and versatility. BENR can be performed by combining nitrification, denitrification, anaerobic ammonium oxidation (ANAMMOX), or organic carbon oxidation. Bioelectrochemical-ANAMMOX (BE-ANAMMOX) is the most promising method for nitrogen removal, as it can directly convert NH 4 + to N 2 and H 2 in one step when the electrode is arranged as an electron acceptor. High-value-added hydrogen can potentially be recovered with efficient nitrogen removal using this concept, maximizing the benefits of BENR. Using alternative electron acceptors, such as electrodes and metal ions, for complete total nitrogen removal is a promising technology to substitute NO 2 - production from NH 4 + oxidation by aeration. However, the requirement of electron donors for NO 3 - reduction, low NH 4 + removal efficiency, and low competitiveness of exoelectrogenic bacteria still remain the main obstacles. The future direction for successful BENR should aim to achieve complete anaerobic NH 4 + oxidation without any electron acceptor and to maximize selectivity in H 2 production. Therefore, the bioelectrochemical pathways and balances between efficient nitrogen removal and high-value-added chemical production should be further studied for carbon and energy neutralities.

Md Monzurul Islam Anoy, Eric Allen Hill, Marci Ranae Garcia et al.

Enzyme and Microbial Technology • 2024

Zhixing Xiao, Dong Wang, Ting Xia et al.

Journal of Water Process Engineering • 2021

Lian-gang Hou, Qi Sun, Zheng-wei Pan et al.

Desalination and Water Treatment • 2023