Gabriel Torrens, Sara Belén Hernández, Juan Alfonso Ayala et al.

mSystems • 2019

The extensive use of β-lactam antibiotics and the bacterial adaptive capacity have led to the apparently unstoppable increase of antimicrobial resistance, one of the current major global health challenges. In the leading nosocomial pathogen Pseudomonas aeruginosa , the mutation-driven AmpC β-lactamase hyperproduction stands out as the main resistance mechanism, but the molecular cues enabling this system have remained elusive until now. Here, we provide for the first time direct and quantitative information about the soluble cell wall-derived fragments accounting for the different levels and pathways of AmpC hyperproduction. Based on these results, we propose a hierarchical model of signals which ultimately govern ampC hyperexpression and resistance.

Ceta Indra Lesmana

Multifinance • 2024

Alleviation poverty, especially among women, is a serious challenge in Indonesia. Finance microsharia emerged as a potential solution. For empowerment, women should approach sharia credit. This article discusses necessary risks noticed, like credit traffic jams, fraud, and risk reputation, in development finance microsharia. Management good risk has become a key continuity business. Apart from that, the importance of education and literacy finance, supporting the government, and partnerships with various parties in increasing the contribution of women to overcome poverty through Sharia credit is highlighted. Utilisation of the potential for zakat, waqf, and Islamic values is also a crucial aspect. Inclusionary and sustainable microfinance models are expected to positively impact women's economic empowerment. With a holistic, inclusive, and sustainable approach, it is hoped that women can play a significant role in alleviating poverty and positively impacting various layers of society in Indonesia.

Li Wang, Xuwei Han, Yu Zhang et al.

Water • 2025

This paper aims to comprehensively explore the performance and influencing factors of the constructed wetland–microbial fuel cell (CW-MFC) system when treating brine with different concentrations. The main objective is to determine how different salinity levels affect the operation and treatment efficiency of the CW-MFC system. The research results show that Bruguiera gymnorrhiza exhibits strong salt tolerance and can be used as a wetland plant for the CW-MFC system. The closed-circuit CW-MFC system with planted plants has the best performance, with a chemical oxygen demand (COD) removal rate of 84.8%, a total nitrogen (TN) removal rate of 68.12%, and a chloride ion (Cl−) removal rate of 29.96%. The maximum power density is 64.79% higher than that of the system without planted plants. The power generation performance of the system first increases and then decreases with the increase in salinity, while the internal resistance keeps decreasing. When the salinity is 2%, the power generation effect is the best, with an average output voltage of 617.3 ± 25.7 mV and a power density of 45.83 mW/m2. The removal rates of COD and TN are inhibited with the increase in salinity, while the removal rate of total phosphorus (TP) is not significantly affected. The microbial community grows well under salt stress, but its structure is different. When the salinity is 1%, the optimal distance between electrodes is 10 cm. Considering the pollutant removal performance, the optimal hydraulic retention time is 3 days, and considering the power generation performance, the optimal hydraulic retention time is 2 days. This research provides important value for improving the performance of the CW-MFC system in treating brine.

Weijie Yang, Chun Zhao, Rui Sun et al.

Frontiers in Sustainable Development • 2025

With the rapid development of the global shipping industry, the pollution of marine ecosystems caused by ship oily sewage is becoming increasingly severe, and the traditional single physical, chemical and biological treatment technology has problems such as high energy consumption, easy secondary pollution and insufficient degradation rate of emulsified oil in the treatment of ship oily water. Therefore, this study focuses on the application of dual-chamber microbial fuel cells (MFCs) in the field of degrading oily sewage from ships. The surface modification of carbon felt anode materials was carried out by chemical activation method, and a two-chamber MFC device was constructed to further explore the effects of different anode materials on the power production performance of MFC and the degradation effect of oily wastewater. The experimental results show that the performance of phosphoric acid-activated carbon felt is particularly outstanding, with a maximum output voltage of 676 mV, which is 25.4% higher than that of unactivated carbon felt and 52.53% higher power density. In terms of the degradation of oily wastewater, it is also significantly better than other modified materials. It has been confirmed that phosphoric acid activation can effectively improve the power generation and degradation efficiency of MFC by increasing the hydroxyl functional group on the surface of carbon felt, improving hydrophilicity and electrochemical properties, and providing a promising technical solution for the treatment of oily wastewater from ships.

Qiong Wan, Xiayin Li, Feng Wang et al.

RSC Advances • 2024

Nitrate-nitrogen pertains to the nitrogen component of the overall nitrate present in a given sample in order to reduce nitrate nitrogen pollution in water, nitrate nitrogen removal methods based on iron–carbon micro-electrolysis have become a key research focus. The process and mechanism of nitrate nitrogen removal by microbial coupling was comprehensively explored in a novel iron–carbon micro-electrolysis (ICME) system. In order to establish the transformation pathway of nitrate nitrogen in water, the transformation paths of nitrate nitrogen in water before and after coupling microorganisms in three groups of continuous flow reaction devices, namely sponge iron (s-Fe0), sponge iron + biochar (s-Fe0/BC) and sponge iron + biochar + manganese sand (s-Fe0/BC/MS), were studied. The morphology and composition changes of sponge iron were analyzed by means of characterization, and the microbial population changes in the three groups were analyzed by high-throughput sequencing. Results showed that the nitrate conversion rate in the s-Fe0, s-Fe0/BC and s-Fe0/BC/MS systems reached 99.48%, 99.57% and 99.36%, respectively, with corresponding ammonia nitrogen generation, rates of 3.77%, 9.34% and 11.24% and nitrogen generation rates of 95.71%, 90.23% and 88.12%. Scanning electron microscopy imaging showed that in the s-Fe0/BC and s-Fe0/BC/MS systems the surface of sponge iron was highly corroded, with granular substances in the corrosion product clusters. X-ray photoelectron spectroscopy analysis found that the relative contents of Fe2O3 in the surface oxides of sponge iron after microbial coupling were 38.02% and 71.27% in the s-Fe0/BC and s-Fe0/BC/MS systems, while the relative Fe3O4 contents were 61.98% and 28.72%, respectively. Microbial high-throughput sequencing analysis revealed that the Chao and Ace index values in the s-Fe0 system were 871.89 and 880.78, while in the s-Fe0/BC system they were 1012.05 and 1017.29, and in the s-Fe0/BC/MS system were 1241.09 and 1198.29, respectively. The relative proportion of Thauera in the s-Fe0, s-Fe0/BC, and s-Fe0/BC/MS systems was 16.76%,14.25% and 10.01%, while the proportion of Acetoanaerobium was 15.36%, 13.27% and 11.11%, and the proportion of Chloroflexi was 0%, 1.11% and 2.18%, respectively. Furthermore, FAPROTAX function annotation found that the expression levels of chemoheterotrophs in the s-Fe0, s-Fe0/BC and s-Fe0/BC/MS systems were 43 316 OTU, 37 289 OTU and 34 205 OTU, while nitrate respiration expression levels were 16 230 OTU, 15 483 OTU and 9149 OTU, with nitrogen respiration expression levels of 16 328 OTU, 15 493 OTU and 9154 OTU, respectively. These findings suggest that nitrate is converted into nitrogen gas and ammonia nitrogen through the actions of the coupled system of sponge iron/biochar/manganese sand and microorganisms. The catalytic effect of MnO2 promotes the conversion of Fe2+ to Fe3+, generating more electrons, allowing denitrifying bacteria to reduce more nitrate nitrogen, effectively coupling the manganese-catalyzed ICME reaction and microbial denitrification. The micro-electrolysis system and the addition of manganese sand enhanced biodiversity within the s-Fe0/BC/MS system. The heterotrophic bacteria Thauera and Acetoanaerobium were the dominant microorganisms in all three systems, although the micro-electrolysis system with added manganese sand significantly reduced the proportion of facultative bacteria Thauera and Acetoanaerobium and promoted the growth of autotrophic Chloroflexi bacteria. The ecological functions of the three systems were mainly nitrate respiration and nitrogen respiration. By comparing the expression levels of nitrate respiration and nitrogen respiration in s-Fe0/BC and s-Fe0/BC/MS systems, it can be seen that the addition of manganese sand reduced microbial activity.

Ahmad Yaseen, Muhammad Rameez Javed, M. Z. U. Rahman et al.

2023 2nd International Conference on Emerging Trends in Electrical, Control, and Telecommunication Engineering (ETECTE) • 2023

Hydrogen gas has tremendous potential as environmentally acceptable energy carrier for vehicles. A cutting-edge technology called a microbial electrolysis cell (MEC) can achieve sustainable and clean hydrogen production from a wide range of biomass and wastewaters. In this work, the research study has been performed on the “textile industry waste” and “dairy manure waste” to analyze the production of hydrogen gas ($H_{2}$) using solar powered MEC. The experiment is performed by adding different percentages of slurry in the textile and dairy waste (substrates) to analyze the production of hydrogen gas in both substrates. The waste is stored in the containers and an anerobic environment is created by using airtight jars. Then, Nitrogen gas is passed, and the containers are fitted with cathode and bio anode. The test samples have been tested after an interval of 20 days through gas chromatography (GC) for the quantization and comparison of the produced gas for both substrates. The obtained results conclude that textile waste produces more hydrogen gas as compared to the other substrate.

Rui Hao Peng, Xiao Jun Xu, Jun Jing An et al.

Applied Mechanics and Materials • 2014

The various affecting factors have been researched in this paper that the leaded wastewater was treated by means of the processing combined Fe-C micro-electrolysis with microbial adsorbent-flocculant. After that combined process, a greater percentage of lead removal was observed. The results showed that under the condition that initial PH of the infall wastewater is 3, the Fe/C mass ratio is 2:1, and the hydraulic retention time is 40 min for the micro electrolysis processing. The PH is 9, the microbial adsorbent-flocculant (ZX-1) dosage is 0.5g/L, and adsorption time is 90 min for subsequent microbial adsorption-flocculation. Under these optimal conditions, the removal rates of lead ion reached 99.958%,and their concentrations in wastewater were 0.042mg/L, which their ion concentrations in the outlet wastewater are under the class III of Surface Water Quality Standards (GB3838-2002) in China. That combined process achieved zero discharge of lead. It has been also showed that Pb ions are mainly removed by Fe/C micro-electrolysis, and the following microbial adsorption-flocculation excels at treating leaded wastewater of low concentration.

Santiago Alvear Méndez, Raúl Bahamonde Soria, Daniel Arboleda et al.

ECSOC 2024 • 2024

: Bioelectrochemical systems are energy-efficient devices that transform chemical energy into electrical energy or synthesize products of interest. These technologies make use of Nafion membranes that function as proton exchangers; however, these separators have some problems, such as fouling. Therefore, this research explores mixed-matrix membranes of chitosan and chitosan derivatives to determine their antimicrobial, anti-fouling, chemical stability and high-water retention properties. The results showed that these membranes could be good candidates to be used as separators in bioelectrochemical systems.

Chun Zhao, Shaojun Zhang, Qianyong Zhang et al.

Scientific Journal of Technology • 2024

In recent years, with the vigorous development of the maritime transportation industry, the resulting problem of ship oil pollution has become increasingly prominent. Efficient and thorough treatment of oily wastewater is the key to solving such problems. Microbial fuel cell (MFC) technology is a promising biological treatment method due to its good degradation effect, no secondary pollution, and energy recovery. In this paper, the discarded corn cob materials in nature were selected for high-temperature carbonization at 350°C, and NaOH was used to modify the modified biochar. Then, the coating technology of Ti3C2 MXene, NbC, WC, TaC and other materials was used to modify the surface of biochar, and the biochar anode material was obtained to construct a double-chamber microbial fuel cell device. The properties of the modified anode biochar materials were studied.

Xuan Guo, Ya Li Zhan, Shao Hui Guo et al.

Advanced Materials Research • 2012

A single-chambered and double-chambered microbial fuel cell (MFC) with refinery oil wastewater as its fuel was constructed respectively to investigate the feasibility of electricity generation and oil pollutants degradation using microbial fuel cell. Results indicated that the refinery oil waste water could be used as fuel in MFCs to generate electricity, the oil pollutants could be degraded by MFCs and the efficiency of double-chambered MFC was better; the main composition of pollutants in the solution influx and effluent of MFCs was changed and processes which were similar to anaerobic fermentation were proceeded.

Kang Ping Cui, Xiao Yan Cao, Song Jin

Advanced Materials Research • 2011

Constructed a two-chambered microbial fuel cell (MFC) and a similar abiotic fuel Cell----Steel fuel cell (SFC), studies the influence of elimination effect about various variables (anode material , size of salt bridge and so on) to the nitrate. The experiment indicated that the elimination efficiency of MFC to the nitrate is 71.46% equally, SFC is 67.99%, and degeneration speed of nitrate is 8mg nitrate/L/day approximately. It has confirmed that MFC and SFC is one highly effective method on elimination nitrate, and the elimination effect of MFC to nitrate is better than SFC.

Zhongyi Liu, M. Minor, P. Morel et al.

Environmental Entomology • 2018

Abstract This study aimed to determine the suitability of several organic waste substrates to be processed by the larvae of the black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) (BSFL) in a value-added bioconversion system. Three types of organic waste (brewer's waste, solid phase of pig manure, and semidigested grass) were tested and compared with a standard larval diet, broll (wheat middling). Larval survival and growth, chemical composition of the resulting prepupae, conversion ratios of nutrients and waste dry matter, and waste reduction rate were measured. Larval survival was high in all tested substrates. Compared with the larvae fed pig manure or semidigested grass, those fed standard diet or brewer's waste showed shorter development time, higher weight gain, and higher prepupal crude protein and crude fat content. BSFL also reduce more dry matter in the standard diet or in brewer's waste than in the other two substrates. On the other hand, larvae fed semidigested grass took 70 d to complete development and suffered fat loss. Thus, we suggest that brewer's waste is the most suitable substrate among the selected wastes for being processed by BSFL, whereas semidigested grass is an unsuitable substrate. We found that lignin had a significantly negative effect on larval growth, and emphasized the importance of applying lignindigesting microorganisms to lignin-rich substrates being converted by BSFL. Moreover, a protein:fat:digestible carbohydrate ratio of 2:1:2 was hypothesized to benefit larval development.

Rajeev Ravindran, S. Hassan, Gwilym A. Williams et al.

Bioengineering • 2018

Agro-industrial waste is highly nutritious in nature and facilitates microbial growth. Most agricultural wastes are lignocellulosic in nature; a large fraction of it is composed of carbohydrates. Agricultural residues can thus be used for the production of various value-added products, such as industrially important enzymes. Agro-industrial wastes, such as sugar cane bagasse, corn cob and rice bran, have been widely investigated via different fermentation strategies for the production of enzymes. Solid-state fermentation holds much potential compared with submerged fermentation methods for the utilization of agro-based wastes for enzyme production. This is because the physical–chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. Recent studies have shown that pretreatment technologies can greatly enhance enzyme yields by several fold. This article gives an overview of how agricultural waste can be productively harnessed as a raw material for fermentation. Furthermore, a detailed analysis of studies conducted in the production of different commercially important enzymes using lignocellulosic food waste has been provided.

T. Fowles, C. Nansen

Food Waste Management • 2019

Fowles and Nansen’s chapter discusses insect-based bioconversion. When insects are mass produced under controlled conditions, they can break down significant quantities of food waste. Further, as the insects consume this waste, they produce multiple valuable commodities, such as insect biomass (proteins, lipids), pharmaceuticals, biofuels, lubricants, and fertilizer from their excrement. This process is called bioconversion and will be a serious contender among food waste treatment options in the coming decades. Insect bioconversion is gaining traction both as a research topic and as a business opportunity. Fowles and Nansen discuss both the need to increase capacity and to maximize the potential benefits of using insects as bioconverters of food waste. They provide both theoretical and practical solutions for expanding insect-based bioconversion to food waste streams.

Wenze Guo, Jiakun Xu, Sheng-Tao Wu et al.

International Journal of Molecular Sciences • 2021

The treatment of environmental pollutants such as synthetic dyes and lignin has received much attention, especially for biotechnological treatments using both native and artificial metalloenzymes. In this study, we designed and engineered an efficient peroxidase using the O2 carrier myoglobin (Mb) as a protein scaffold by four mutations (F43Y/T67R/P88W/F138W), which combines the key structural features of natural peroxidases such as the presence of a conserved His-Arg pair and Tyr/Trp residues close to the heme active center. Kinetic studies revealed that the quadruple mutant exhibits considerably enhanced peroxidase activity, with the catalytic efficiency (kcat/Km) comparable to that of the most efficient natural enzyme, horseradish peroxidase (HRP). Moreover, the designed enzyme can effectively decolorize a variety of synthetic organic dyes and catalyze the bioconversion of lignin, such as Kraft lignin and a model compound, guaiacylglycerol-β-guaiacyl ether (GGE). As analyzed by HPLC and ESI-MS, we identified several bioconversion products of GGE, as produced via bond cleavage followed by dimerization or trimerization, which illustrates the mechanism for lignin bioconversion. This study indicates that the designed enzyme could be exploited for the decolorization of textile wastewater contaminated with various dyes, as well as for the bioconversion of lignin to produce more value-added products.

E. Park, J. Ha, S. Lim et al.

Journal of Dairy Science • 2021

This study developed postbiotics with whey bioconversion product produced by Enterococcus faecalis M157 KACC 81148BP, and mixed whey bioconversion products produced by E. faecalis M157 KACC 81148BP and Lactococcus lactis ssp. lactis CAU2013 KACC 81152BP to alleviate periodontitis (PD) and to improve gut health. The powdered whey bioconversion product (EF) produced by E. faecalis M157 KACC 81148BP, mixed whey bioconversion products (EF+LL) from E. faecalis M157 KACC 81148BP and L. lactis CAU2013 KACC 81152BP, and phosphate-buffered saline (PBS; control) were administered orally to PD-induced rats for 8 wk. Infiltration of inflammatory cells and epithelial proliferation in periodontal tissue were found in control, but the lesions were reduced in PD+EF group (administration of EF to PD-induced rats), and no lesions were observed in PD+EF+LL group (administration of EF+LL to PD-induced rats). The bone loss volumes in PD+EF and PD+EF+LL groups were lower than in control. Cytokine production levels related to inflammation were lower and antioxidative stress markers were higher in PD+EF and PD+EF+LL groups than in control for both periodontal tissue and gut. The ratios of Lactobacillus spp. in gut microbiome of PD+EF and PD+EF+LL groups were higher than in control. These results indicate that the whey bioconversion product produced by E. faecalis M157 KACC 81148BP, and mixed whey bioconversion products produced by E. faecalis M157 KACC 81148BP and L. lactis CAU2013 KACC 81152BP are effective on relieving periodontitis and improving the gut health.

Ja Kyong Ko, T. Enkh-Amgalan, Gyeongtaek Gong et al.

GCB Bioenergy • 2020

Lignocellulosic biomass has considerable potential for the production of fuels and chemicals as a promising alternative to conventional fossil fuels. However, the bioconversion of lignocellulosic biomass to desired products must be improved to reach economic viability. One of the main technical hurdles is the presence of inhibitors in biomass hydrolysates, which hampers the bioconversion efficiency by biorefinery microbial platforms such as Saccharomyces cerevisiae in terms of both production yields and rates. In particular, acetic acid, a major inhibitor derived from lignocellulosic biomass, severely restrains the performance of engineered xylose‐utilizing S. cerevisiae strains, resulting in decreased cell growth, xylose utilization rate, and product yield. In this study, the robustness of XUSE, one of the best xylose‐utilizing strains, was improved for the efficient conversion of lignocellulosic biomass into bioethanol under the inhibitory condition of acetic acid stress. Through adaptive laboratory evolution, we successfully developed the evolved strain XUSAE57, which efficiently converted xylose to ethanol with high yields of 0.43–0.50 g ethanol/g xylose even under 2–5 g/L of acetic stress. XUSAE57 not only achieved twofold higher ethanol yields but also improved the xylose utilization rate by more than twofold compared to those of XUSE in the presence of 4 g/L of acetic acid. During fermentation of lignocellulosic hydrolysate, XUSAE57 simultaneously converted glucose and xylose with the highest ethanol yield reported to date (0.49 g ethanol/g sugars). This study demonstrates that the bioconversion of lignocellulosic biomass by an engineered strain could be significantly improved through adaptive laboratory evolution for acetate tolerance, which could help realize the development of an economically feasible lignocellulosic biorefinery to produce fuels and chemicals.

Jaewoo Son, J. H. Jang, I. H. Choi et al.

Microbial Cell Factories • 2021

Background trans -cinnamic acid ( t -CA) is a phenylpropanoid with a broad spectrum of biological activities including antioxidant and antibacterial activities, and it also has high potential in food and cosmetic applications. Although significant progress has been made in the production of t -CA using microorganisms, its relatively low product titers still need to be improved. In this study, we engineered Corynebacterium glutamicum as a whole-cell catalyst for the bioconversion of l -phenylalanine ( l -Phe) into t -CA and developed a repeated bioconversion process. Results An expression module based on a phenylalanine ammonia lyase-encoding gene from Streptomyces maritimus (SmPAL), which mediates the conversion of l -Phe into t -CA, was constructed in C. glutamicum . Using the strong promoter P H36 and ribosome binding site (RBS) (in front of gene 10 of the T7 phage), and a high-copy number plasmid, SmPAL could be expressed to levels as high as 39.1% of the total proteins in C. glutamicum . Next, to improve t -CA production at an industrial scale, reaction conditions including temperature and pH were optimized; t -CA production reached up to 6.7 mM/h in a bioreactor under optimal conditions (50 °C and pH 8.5, using NaOH as base solution). Finally, a recycling system was developed by coupling membrane filtration with the bioreactor, and the engineered C. glutamicum successfully produced 13.7 mM of t -CA (24.3 g) from 18.2 mM of l -Phe (36 g) and thus with a yield of 75% (0.75 mol/mol) through repetitive supplementation. Conclusions We developed a highly efficient bioconversion process using C. glutamicum as a biocatalyst and a micromembrane-based cell recycling system. To the best of our knowledge, this is the first report on t -CA production in C. glutamicum , and this robust platform will contribute to the development of an industrially relevant platform for the production of t -CA using microorganisms.

Harish Karthikeyan Ravi, A. Degrou, J. Costil et al.

Processes • 2020

Each year, the food supply chain produces more than 1.3 billion tons of food and agricultural waste, which poses serious environmental problems. The loss of the massive quantity of secondary and primary metabolites retrievable from this resource is a significant concern. What if there is a global solution that caters to the numerous problems arising due to the humongous volume of waste biomass generated in every part of the world? Insects, the tiny creatures that thrive in decaying organic matter, which can concentrate the nutrients present in dilute quantities in a variety of by-products, are an economically viable option. The bioconversion and nutritional upcycling of waste biomass with insects yield high-value products such as protein, lipids, chitin and frass. Insect-derived proteins can replace conventional protein sources in feed formulations. Notably, the ability of the black soldier fly (BSF) or Hermetia illucens to grow on diverse substrates such as agri-food industry side streams and other organic waste proves advantageous. However, the data on industrial-scale extraction, fractionation techniques and biorefinery schemes for screening the nutritional potential of BSF are scarce. This review attempts to break down every facet of insect processing and analyze the processing methods of BSF, and the functional properties of nutrients obtained thereof.

Qihong Lu, Yongyi Liang, Wenwen Fang et al.

Environmental Science & Technology • 2021

Surface sediments of polluted urban rivers can be a reservoir of hydrophobic persistent organic pollutants (POPs). In this study, we comprehensively assessed the contamination of two groups of POPs, that is, polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), in 173 black-odorous urban rivers in China. Spatial distribution of PCBs and PBDEs showed similar patterns but very different contamination levels in surface sediments, that is, average concentrations of 10.73 and 401.16 ng/g dw for the ∑PCBs and ∑PBDEs, respectively. Tetra-/di-CBs and deca-BDE are major PCBs and PBDEs and accounted for 59.11 and 95.11 wt % of the ∑PCBs and ∑PBDEs, respectively. Compared with the persistence of PBDEs, the EF changes of chiral PCBs together with previous cultivation evidence indicated indigenous bioconversion of PCBs in black-odorous urban rivers, particularly the involvement of uncharacterized Dehalococcoidia in PCB dechlorination. Major PCB sources (and their relative contributions) included pigment/painting (25.36%), e-waste (22.92%), metallurgical industry (13.25%), and e-waste/biological degradation process (10.95%). A risk assessment indicated that exposure of resident organisms in urban river sediments to deca-/penta-BDEs could pose a high ecological risk. This study provides the first insight into the contamination, conversion and ecological risk of PCBs and PBDEs in nationwide polluted urban rivers in China.

C. Scieuzo, Antonio Franco, R. Salvia et al.

Insect Science • 2022

Bioconversion is a biological process by which organic materials are converted into products with higher biological and commercial value. During its larval stage the black soldier fly Hermetia illucens is extremely voracious and can feed on a wide variety of organic materials. To study the impact of different fruit byproducts on the insect's growth, final larval biomass, substrate reduction, bioconversion parameters, and larval nutritional composition, 10 000 black soldier fly larvae (BSFL) were reared on 7.0 kg of one of three substrates (strawberry, tangerine, or orange) or on a standard diet as a control. The results highlight that BSFL can successfully feed and grow on each of these diets, though their development time, growth rate, and final biomass were differently impacted by the substrates, with strawberry being the most suitable. The lipid and protein contents of BSFL were similar among larvae fed on different substrates; however, major differences were detected in ash, micronutrient, fiber, fatty acid, and amino acid contents. Overall, the results indicate that fruit waste management through the BSFL bioconversion process represents a commercially promising resource for regional and national agrifood companies. Our study offers new perspectives for sustainable and environmentally friendly industrial development by which fruit byproducts or waste might be disposed of or unconventionally enhanced to create secondary products of high biological and economic value, including BSFL biomass as animal feed or, in perspective, as alternative protein source for human nutrition.

W.W. Clarkson, X. Xiao

Water Science and Technology • 2000

Anaerobic bioconversion of newsprint and waste office paper was performed in bench-scale reactors with three inocula sources: landfill, rumen, and anaerobic digester. Office paper bioconversion was nearly complete within 20 days but continued for about 165 days with methane yield efficiencies ranging from 71–85% of potential chemical oxygen demand (COD) conversion. Average newsprint methane conversion efficiencies ranged from 32–41% of total COD under strictly anaerobic conditions for 300 days. Mass balance calculations revealed that more than 80% of newsprint cellulose was biodegraded. The apparent limiting factor for anaerobic bioconversion of newsprint was the physical association between lignin and cellulose. After proper acclimation, the three inocula tested equally well for methane production under strictly anaerobic conditions. Testing of ground, shredded strips, and whole paper pieces showed no effects of feedstock size on bioconversion rate or extent. Alkali pretreatment with NaOH concentration up to 10% significantly improved newsprint biodegradability. Treatment for longer duration or at elevated temperatures increased the solubilization of lignin, but did not improve bioconversion of newsprint to methane. Neutralizing treated samples with carbon dioxide gave higher methane yields compared to sulfuric or hydrochloric acids, suggesting that digester neutralization could be combined with biogas scrubbing.

Jens Boyen, Patrick Fink, Christoph Mensens et al.

bioRxiv (Cold Spring Harbor Laboratory) • 2019

Abstract By 2100, global warming is predicted to significantly reduce the capacity of marine primary producers for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis. Primary consumers such as harpacticoid copepods (Crustacea) might mitigate the resulting adverse effects on the food web by increased LC-PUFA bioconversion. Here, we present a high-quality de novo transcriptome assembly of the copepod Platychelipus littoralis , exposed to changes in both temperature (+3°C) and dietary LC-PUFA availability. Using this transcriptome, we detected multiple transcripts putatively encoding for LC-PUFA-bioconverting front-end fatty acid desaturases and elongases, and performed phylogenetic analyses to identify their relationship with sequences of other (crustacean) taxa. While temperature affected the absolute fatty acid concentrations in copepods, LC-PUFA levels remained unaltered even when copepods were fed a LC-PUFA-deficient diet. While this suggests plasticity of LC-PUFA bioconversion within P. littoralis , none of the putative front-end desaturase or elongase transcripts were differentially expressed under the applied treatments. Nevertheless, the transcriptome presented here provides a sound basis for future ecophysiological research on harpacticoid copepods.

Ramu Selvam, Paul Olusegun Bankole, PhD et al.

Research Square • 2023

Abstract Vermistabilization of the textile industrial sludge (TS) was carried out on a pilot scale with the help of the earthworm Eudrilus eugeniae . TS was mixed with cow dung (CD) in a ratio of 1:1 and thereafter subjected to vermicomposting for 90 days. Earthworms’ weights increased significantly by 55% in the feed mixture within 45 days of inoculation. Physicochemical parameters significantly reduced during vermicomposting (pH, EC, TDS, TOC, and NH 3 -N) whereas TKN, TP, TK were found to be significantly elevated in the final product. Except for Mn and Zn, the addition of amendments increased earthworm activity, which greatly reduced the content of heavy metals (Al, As, Cd, Cu, Pb, Ni, Cr, Mo, and Hg). Germination index (100%) of Brassica nigra was recorded in the feed mixture. The shoot and root length ranged from 2.26 and 4.36 after 90 days. Genotoxicity analysis revealed a 9.45–10.40% reduction in aberration frequencies of vermistabilized extracts in comparison with control (extracts of TS and CD only). The present study concluded that the deployment of vermistabilized extracts looks promising as a sustainable waste disposal management technology.

Chaichi Devi, Meena Khwairakpam

New Generation of Organic Fertilizers • 2022

Organic fertilizers are alternative to chemicals used in agriculture which enhance soil quality, prevent harmful chemicals entering into food chain, improve health and contribute to sustainable future socially, economically and ecologically. Vermicompost is a nutrient-rich organic fertilizer which promotes plant growth and improves soil quality. Vermicomposting is an economically feasible and environment friendly technology in which organic wastes are bio-converted into value added product and various organic wastes are used in this process. Terrestrial weeds are the plant species which grow on land and invasive in nature. These plants are responsible for various nuisances in the environment, agriculture and society. The weed biomass generated after various management methods are considered as organic waste. The terrestrial weed biomass is a possible option for the production of vermicompost. In this chapter scope of vermicompost for sustainable agriculture, the vemicomposting mechanism and the bioconversion of terrestrial weed biomass into vermicompost have been discussed.

Aneela Taj, Nusrat Jamil

Biomolecules • 2018

The biochemical potential of pathogenic bacteria may cause alteration in the neurophysiological environment; consequently, neuroendocrine and immune responses of the host are modulated by endogenously produced metabolic products of neuropathogenic bacteria. The present study was designed to detect the derived biogenic amines in spent culture media of Bacillus cereus (Bc), Clostridium tetani (Ct), Listeria monocytogenes (Lm), and Neisseria meningitidis (Nm). Overnight grown culture in different culture media i.e., Nutrient broth (NB), Luria basal broth (LB), Brain Heart Infusion broth (BHI), and human serum supplemented RPMI 1640 medium (RPMI) were used to prepare filter-sterilized, cell-free cultural broths (SCFBs) and subjected to high performance liquid chromatography with electrochemical detection (HPLC-EC) along with the control SCFBs. Comparative analysis of biogenic amines in neuropathogenic bacterial SCFBs with their respective control (SCFB) revealed the complete degradation of dopamine (DA) into its metabolic products by Bc, Ct, and Nm, whereas Lm showed negligible degradation of DA. A relatively high concentration of 5-hydroxyindol acetic acid (5HIAA) by Bc in NB and LB indicated the tryptophan metabolism by the serotonin (5HT) pathway. Our study suggests that microbial endocrinology could help unravel new perspectives to the progression of infectious diseases.

R. Pandey, T. Sohail, A. I. Ajibona et al.

57th U.S. Rock Mechanics/Geomechanics Symposium • 2023

ABSTRACT The total annual US consumption of natural gas is expected to surpass 40 trillion cubic feet in the coming years. Microbially enhanced coalbed methane (MECBM) aims to replicate naturally occurring microbial pathways to generate methane from in-situ coal. In a basic gamut of lab-characterization experiments investigating properties of coal as a reservoir, it was revealed microbial treatment of coal results in swelling of the coal matrix. Bio-strains in the matrix result in changes in connected porosity, and its stress-state which governs the flow behavior throughout the life of the producing reservoir. Use of molecular dynamics (MD) enables us to utilize the bio-strain data to understand the dynamic stress-state development in a MECBM reservoir. The Wiser coal molecule was used as the representative molecule, whose reactive potential was minimized using PCFF. The stable MD system enables application of strain, which enables the analysis of internal stresses. Results indicated internal stresses developed during bioconversion exceeded the Von Mises failure criterion for the sample tested in the laboratory under hydrostatic pressure (0.2 MPa). However, the internal stresses for sample under in-situ stress regimes was suppressed, far from the tensile failure conditions. INTRODUCTION AND BACKGROUND There has been a sustained increase in the demand for clean energy sources such as hydrogen and methane, especially as the world works towards meeting sustainable climate goals (Jun et al., 2016). Natural gases like methane have lower carbon footprint, as it generates approximately 50% of the carbon when compared to burning oil and coal for electricity generation (Tollefson, 2012). As such, natural gases are set to go up in production rates to meet environmental demands. Recent data indicates that the world demand for natural gas is expected to increase till 2045, with a significant portion of this demand to be met by increasing production from unconventional resources, such as coalbed methane (CBM) (Birol, 2017; Gonzales, 2021; IEA, 2022). CBM refers to naturally occurring methane extracted from coal and coal seams, which is an unconventional source of natural gas (Haldar, 2018). However, to meet the increasing demand for natural gas, researchers have made efforts to find ways to increase the production of coalbed methane. One such method is to replicate the natural production of methane formed by microbial breakdown of organic components present in coal (Flores et al., 2008; Strapoć et al., 2008; Midgley et al., 2010; Penner et al., 2010). This process of producing microbial methane from coal is referred to as Microbially Enhanced Coalbed Methane (MECBM) (Scott, 1999).

Chen Chang, Bo Liu, Yihong Bao et al.

Research Square • 2020

Abstract Background Phenylpropanoid including raspberry ketone, is a kind of important natural plant product and widely used in pharmaceuticals, chemicals, cosmetics, and healthcare products. Bioproduction of phenylpropanoid in Escherichia coli and other microbial cell factories is an attractable approach considering the low phenylpropanoid contents in plants. However, it is usually difficult to produce high titer phenylpropanoid production when fermentation using glucose as carbon source. Developing novel bioprocess using alternative sources might provide a solution to this problem. In this study, typical phenylpropanoid raspberry ketone was used as the target product to develop a biosynthesis pathway for phenylpropanoid production from fatty acids, a promising alternative low-cost feedstock. Results A raspberry ketone biosynthesis module was developed and optimized by introducing 4-coumarate-CoA ligase (4CL), benzalacetone synthase (BAS), and raspberry ketone reductase (RZS) in Escherichia coli strains CR1-CR4. Then strain CR5 was developed by introducing raspberry ketone biosynthesis module into a fatty acids-utilization chassis FA09 to achieve production of raspberry ketone from fatty acids feedstock. However, the production of raspberry ketone was still limited by the low biomass and unable to substantiate whole-cell bioconversion process. Thus, a process by coordinately using fatty-acids and glycerol was developed. In addition, we systematically screened and optimized fatty acids-response promoters. The optimized promoter Pfrd3 was then successfully used for the efficient expression of key enzymes of raspberry ketone biosynthesis module during bioconversion from fatty acids. The final engineered strain CR8 could efficiently produce raspberry ketone repeatedly using bioconversion from fatty acids feedstock strategy, and about 291.3 mg/L raspberry ketone was produced. Conclusion Metabolically engineered Escherichia coli strains were successfully developed for raspberry ketone production from fatty acids using several strategies, including optimization of bioconversion process and fine-tuning key enzyme expression. This study provides an essential reference to establish the low-cost biological manufacture of phenylpropanoids compounds.

Sergii Shamanskyi, Sergii Boichenko, Lesia Pavliukh

ScienceRise • 2020

The object of research: the process of wastewater treatment using bioconversion for subsequent motor fuel production. Investigated problem: improving the efficiency of bioconversion process for biofuel production with simultaneous wastewater treatment by removing nitrogen and phosphorous compounds. The main scientific results: providing the possibility of biofuel production with energy and economic inefficiency. It is done by combining the process of motor biofuel production with the process of treating wastewater from biogenic elements makes it perspective for commercial use. Traditional technology for the production of motor biofuels from microalgae includes cultivation, harvesting, dehydration and drying of biomass, extraction of oils from them and subsequent production of methyl esters and glycerol. Such technology is often not economically effective. It requires significant amount of energy for carrying out all necessary processes. In addition, it requires significant expenditures of water and nutrients. The use of nutrient-rich wastewater as a culture medium for the cultivation of microalgae allows not only to reduce costs, but also to purify wastewater from nitrogen and phosphorus compounds, which makes this process economically effective. The area of practical use of the research results: Sewage and gray water treatment plants. Industrial and agricultural effluents treatment plants. Different types of enterprises, which have wastewater enriched with nitrogen and phosphorous compounds. Innovative technological product: The technology of microalgae cultivation using wastewater as a culture medium. The technology allows effectively purifying used wastewaters from nitrogen and phosphorous compounds with no waste at the end. Scope of the innovative technological product: Improved technology of motor biofuel production with simultaneous wastewater purification, which is economically effective and environmentally safe.

Alfonso Timoneda, Arian Amirvaresi, Reza Ovissipour

bioRxiv (Cold Spring Harbor Laboratory) • 2024

Abstract Developing low-cost media is one of the major challenges in the cellular agriculture domain. Thus, this study aimed to develop low-cost media for cell-cultivated seafood using gut-microbial community-assisted fermentation. Black soldier fly larvae ( Hermetia illucens ) were used as the substrate and exposed to gut microbial communities isolated from Blue catfish ( Ictalurus furcatus ). In the first step, BSFL slurry was subjected to enzymatic digestion, using pepsin and trypsin to mimic animal digestive processes. The results showed a 2.8% degree of hydrolysis after digestion with pepsin and an additional 5.9% after digestion with trypsin. In the second step, two fermentation approaches were tested, including the direct addition of gut homogenate to the hydrolysates (fermentation A) and the establishment of microbial cultures from the gut homogenate before fermentation (fermentation B). Both fermentations resulted in similar protein content and degree of hydrolysis. Fermentation led to a decrease in species richness, with the loss of important chitinase and protease-producing genera such as Pseudomonas and Clostridiaceae. However, there was an increase in Paraclostridium and members of the Enterobacteriaceae. In addition, the effect of fermented hydrolysates from BSFL on the proliferation of zebrafish embryo fibroblasts was tested in comparison to fetal bovine serum (FBS) in in vitro cell cultivation. Lower concentrations of FBS resulted in decreased cell density and altered cell morphology. The supplementation of hydrolysate B at high peptide concentrations had cytotoxic effects on the cells, while at lower peptide concentrations, it improved cell proliferation only in cultures with 2.5% FBS.

Kamran Malik, Chunjie Li, Adnan Arshad

• 2025

Background: Appropriate bioprocessing of lignocellulosic materials into ethanol could address the world’s insatiable appetite for energy while mitigating greenhouse gases. Bioethanol is an ideal gasoline extender and is widely used in many countries in blended form with gasoline at specific ratios to improve fuel characteristics and engine performance. Finding a suitable microbial agent for the efficient conversion of lignocellulose is still an active field of study. Objective: To enhance the bioethanol production with effective lignin degradation and utilization of pentose and hexose sugars in an economical way. Methods: Ryegrass (Lolium perenne L.) biomass was the substrate. Microbial strains Bacillus mobilis, Bacillus velezensis, and Bacillus cereus, were isolated, identified, determined for their lignin degradation capability, and used as pretreatment agents for the lignin degradation. Various modern spectroscopic analyses, ligninolytic activity, sugar estimation, enzymatic hydrolysis, and liquefaction and fermentation process were conducted. The final data was statistically validated with post-hoc Tukey test, R software and SPSS Statistics 26.Results: The proximate and ultimate analyses of raw biomass showed that it comprised of total solids 96.54%, volatile solids 92.82%, carbon 48.22%, and sulphur 0.28%. After the application of bacterial pretreatments, the lignin content was considerably reduced to 6.78%, and the cellulose share increased to 57.31%. The LiP and MnP like activity was highest in alkaline lignin culture source with an amount of 0.67 ± 0.1 U/mL and 1.03 ± 0.08 U/mL, respectively. The optimum sugar utilization efficiency was reached at 93.46 %, with the highest bioethanol production of 0.51 g/g and 85.78 % bioethanol yield after the anaerobic fermentation.Conclusion: In this study, successful delignification of the ryegrass biomass was achieved by bacterial pretreatments and maximum bioethanol was produced. The integration of bacterial pretreatments and C5 and C6 sugar utilizing microbial strains could enhance the commercial bioethanol production. The ryegrass biomass was selected since it is a common agricultural waste in China. The transformation of this biomass into industrial products like ethanol, would not only utilize waste but also accord to environmental safety. However, to meet global energy demand further studies to develop sustainable and cost-effective approaches are still required.

Nalini Nalini, D. Jeyachitra

INDIAN JOURNAL OF APPLIED RESEARCH • 2025

The present study is aimed with an objective to produce bio ethanol from agrowaste. Paddy straw bio mass is collected from agricultural land and utilized as feed stock for ethanol production after subjecting them to acid, alkali and enzymatic pre treatments. Two bacterial strains (R2 and R3) and one yeast strain (S6) were isolated from soil samples collected from paddy field dumped with straw and from sugarcane bagasse dumped soil. In addition to this, cellulose degrading bacteria strain (C1) is isolated to carry out the enzymatic pre treatment. Based on the fermentation efficiency tested, S6, R2 and R3 strains were inoculated in eight different pretreated biomass filtrate medium for fermentation process. Ethanol concentration were quantified at a period of five days interval up to twenty days. Ethanol concentration increased from 5th day to 20th day and 5th day to 15th day in yeast and bacteria inoculated medium. After recovery through distillation, yeast strain S6 inoculated medium is reported with more concentration of ethanol in alkali pre treated bio mass filtrate medium. There is no significant difference between alkali pre treated biomass and combined alkali & enzyme pre treated bio mass inoculated with yeast strain at 0.001 level of significance. The overall product extracted is in the range of 5-12%. Ethanol extracted from yeast S6 is reported to have disinfectant effect against bacteria. FTIR spectrum analysis reported the presence of similar peaks to that of standard ethanol

Zhina Lian, Xin Zhou

Fermentation • 2024

Lignocellulosic biomass (LCB) offers numerous advantages as a sustainable energy resource, such as its abundance, degradability, environmental compatibility, non-toxic nature, and cost-effectiveness [...]

, Kerisha Raghunandan

• 2013

The continual growth of the global biodiesel industry has resulted in a proportional increase in crude glycerol production. The by-production of glycerol waste during the manufacture of biodiesel has, with recent research, proven to hold use as a feedstock for the production of several commodity chemicals. The conversion of glycerol may be carried out by both chemical as well as biological means. The biological conversion of glycerol surpasses chemical conversion with respect to higher yield and selectivity, normal reaction conditions and the use of cheaper biological catalysts. Many microorganisms are known to convert glycerol to different value added products. This study involved the isolation of bacteria from soil and crude glycerol from a local biodiesel plant. Isolates were then used to convert crude glycerol supplemented with salts and a nitrogen source into commercially viable products. Isolates which successfully degraded glycerol were then identified via 16S PCR. A strain of Klebsiella pneumoniae, which is a known producer of 1,3-propanediol (1,3-PDO), was isolated from soil and two strains of Sphingomonas sp., which is a known gellan producer, was isolated from biodiesel waste. Gellan is an exopolysaccharide used in the food, cosmetic and pharmaceutical industries sold commercially as a product known as Gelrite or Gelzan while 1,3-PDO is an important component of fuels and polyesters (used widely in the petroleum industry) and is currently chemically produced. Using crude glycerol for producing 1,3-PDO is a good solution from an economic as well as ecological point of view. K. pneumoniae, Sphingomonas psueudosanguinis and Sphingomonas yabuuchiae were subjected to a series of shake flask fermentations in order to determine optimal growth conditions. This microoganism was able to successfully produce significant amounts of 1,3-PDO and lactic acid using crude glycerol (80 g/l), without pre-treatment (37 and 6.8 g/l respectively). S psueudosanguinis and S. yabuuchiae were both able to produce two of the highest amounts of gellan gum than that reported by other studies using crude glycerol (80 g/l) as a sole carbon source in a minimal medium (50.9 and 52.6 g/l respectively).

Carlos Martín

Agronomy • 2021

Decreasing the dependence on fossil resources as raw materials for the production of fuels, platform chemicals, and commodities is an imperative requirement of today’s industry and society in order to alleviate the threats related to climate change [...]

, Z. Benattouche, K. Benamar et al.

Romanian Journal of Infectious Diseases • 2024

Background. The Maillard product was prepared from four model systems (aminoacids-glucose) at pH 10 and 110°C for 120 mn. Aim. This study aims to investigate the antimicrobial potential of different Maillard products and their hemolytic effect. Methods. Maillard products were prepared by four models (Gly-glu), (Val-glu), (Try-glu) and (Asp-glu). The prepared Maillard product antimicrobial activity was tested at concentration of 0.24, 0.48, 0.97, 1.95 and 3.90 mg/ml against Escherichia coli ATCC 25923, Staphylococcus aureus ATCC 25922, Candida albicans and Fusarium graminearum) and hemolytic activity. Results. The inhibition diameter of the test isolate by-products from 4 models of Maillard reaction (Gly-glu), (Val-glu), (Try-glu), and (Asp-glu) ranged from 15±0mm to 28.3±0.4mm. The minimum inhibitory concentration (MIC) ranged from 0.97 mg/ml to 1.95 mg/ml for only two test isolates. Maillard reaction products also showed a similar effect of growth inhibition compared with antibiotics frequently used for treating bacterial infections. An evaluation of the hemolytic activity of the Maillard products on erythrocytes showed a low hemolysis rate of the order of 12.09%. Conclusion. The Maillard products tested had vigorous antimicrobial activity against some pathogenic microorganisms. The Maillard reaction product can be used as a narrow-spectrum antibiotic.

Valeriy Bekmuradov

• 2021

Production of biofuel such as ethanol from lignocellulosic biomass is a beneficial way to meet sustainability, energy security, and environmental goals. Lignocellulosic biomass such as source-separated organic (SSO) waste is particularly attractive since it is widely available, often at a negative cost, reduce the land depletion from using food-based biomass for ethanol production and reduce the amount of generated waste. Therefore, in order to meet the future fuel demands and cope with increasing volume of municipal waste this study was a first attempt to use SSO as a feedstock for ethanol production. The main objectives of the study were: a) to compare standard and modified celluloseorganic- solvent-based lignocellulosic fractionation (COSLIF) pretreatment of SSO waste for ethanol production in terms of enzyme savings, sugar formation and ethanol yields; b) to produce ethanol from SSO by using modified COSLIF pretreatment and fermentation with two different recombinant strains: Z. mobilis 8b and S. cerevisiae DA2416; and c) to develop experimental kinetic model capable of predicting behavior of batch SSCF on SSO waste with different SSO substrate concentrations using Berkeley Madonna program. Based on the obtained results, it was found that SSO is an excellent feedstock material for ethanol conversion. The efficiency of modified COSLIF pretreatment was improved by 20% compared to standard method using ethanol washing of pretreated SSO samples during the experimental procedures instead of acetone. On average, glucose yield from SSO samples pretreated by modified COSLIF was about 90% compared to 10% for untreated samples. S. cerevisiae DA2416 outperformed Z. mobilis 8b on ethanol yields during the fermentation process, with 0.50 g ethanol/g potential sugar fed on SSO in less than 5 days, with a 96% cellulose conversion, totalling in 150 g/L ethanol produced. A kinetic model with newly integrated values of experimentally defined SSO feedstock constants was proven to predict the ethanol yield accurately with substrate concentration ranges of 20 g/L - 50 g/L. Model prediction at higher substrate concentration (e.g. 100 g/L) deviated from the experimental values, suggesting that ethanol inhibition is a major factor in bioethanol conversion.

Ryan Davis, Mary Biddy, Eric C. D. Tan et al.

• 2013

In support of the Bioenergy Technologies Office, the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL) are undertaking studies of biomass conversion technologies to identify barriers and target research toward reducing conversion costs. Process designs and preliminary economic estimates for each of these pathway cases were developed using rigorous modeling tools (Aspen Plus and Chemcad). These analyses incorporated the best information available at the time of development, including data from recent pilot and bench-scale demonstrations, collaborative industrial and academic partners, and published literature and patents. This technology pathway case investigates the biological conversion of biomass derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot scale demonstrations at NREL. Technical barriers and key research needs have been identified that should be pursued for the pathway to become competitive with petroleum-derived gasoline, diesel and jet range hydrocarbon blendstocks.

L. Lynd, G. Beckham, A. Guss et al.

Energy & Environmental Science • 2022

Hybrid processes, featuring biological conversion of lignocellulose to small molecules followed by chemo-catalytic conversion to larger molecules suitable for difficult-to-electrify transport modes, are a promising route to biomass-derived fuels in demand for climate stabilization.

J. Hyeon, S. W. Kim, Chulhwan Park et al.

Chemical Communications • 2015

An enzyme complex for biological conversion of CO to CO2 was anchored on the cell surface of the CO2-utilizing Ralstonia eutropha and successfully resulted in a 3.3-fold increase in conversion efficiency. These results suggest that this complexed system may be a promising strategy for CO2 utilization as a biological tool for the production of bioplastics.